Introduction to spin label electron paramagnetic resonance spectroscopy of proteins

Demystifying EPR: A Rookie Guide to the Application of Electron Paramagnetic Resonance Spectroscopy on Biomolecules

Electron Paramagnetic Resonance (EPR) spectroscopy, also known as Electron Spin Resonance(ESR) especially among physicists, is a strong and versatile spectroscopic method forinvestigation of paramagnetic systems, i.e. systems like free radicals and most transition metalions, which have unpaired electrons. The sensitivity and selectivity of EPR are notable andintriguing as compared to other spec...

Electron Paramagnetic Resonance Spectroscopy

Electron-spin-resonance/electron-paramagnetic-resonance spectroscopy of iron-sulphur enzymes.

The conjugated iron-sulphur proteins are enzymes that contain iron-sulphur clusters, which are paramagnetic in certain oxidation states. Numerous enzymes of this type have been isolated (Palmer, 1975; Cammack, 1979; Yoch & Carithers, 1979), and whole books have been devoted to them (Lovenberg, 1977; Spiro, 1982). Most of them (aconitase being an exception) are oxidoreductases. By using e.p.r. (...

Exploring intrinsically disordered proteins using site-directed spin labeling electron paramagnetic resonance spectroscopy

Proteins are highly variable biological systems, not only in their structures but also in their dynamics. The most extreme example of dynamics is encountered within the family of Intrinsically Disordered Proteins (IDPs), which are proteins lacking a well-defined 3D structure under physiological conditions. Among the biophysical techniques well-suited to study such highly flexible proteins, Site...

demystifying epr: a rookie guide to the application of electron paramagnetic resonance spectroscopy on biomolecules

electron paramagnetic resonance (epr) spectroscopy, also known as electron spin resonance(esr) especially among physicists, is a strong and versatile spectroscopic method forinvestigation of paramagnetic systems, i.e. systems like free radicals and most transition metalions, which have unpaired electrons. the sensitivity and selectivity of epr are notable andintriguing as compared to other spec...